Our new Obvious+ tissue clearing technique is the one strategy that delipidates samples without any alter in morphology and with nominal influence on structural integrity.
Megatome is really a vibrating microtome designed to portion a wide selection of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome permits large-throughput tissue sectioning with uniform surface profile, and nominal tissue destruction and knowledge loss.
Antibodies may possibly take weeks to diffuse through just a few millimeters of tissue, which has a steep labeling gradient from surface area to Main.
Megatome is made for accuracy: the blade vibrates at a greater frequency and larger amplitude array than other microtomes, and includes a exceptional deflection control system.
SE utilizes a rotational electrical industry to disperse extremely electromobile molecules (for example antibodies or surfactant micelles) in the course of a porous sample devoid of detrimental electrically billed structures inside the tissue. This allows 2-4 day clearing of intact organs,
task to generate an aggregated Javadoc. With this occasion, javadocTask need to megatomi.com be established to allJavadoc in order that the correct documentation is built before creating the docset.
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It’s time for you to improve your microtome to Megatome. With correct superior-frequency slicing for an unmatched choice of sample measurements and types – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for varied programs.
Completely delipidate whole mouse brains or comparably sized samples in only one day with SmartBatch+, or in a single week with our passive clearing package.
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Protect avoids the variability of hydrogel embedding and the data loss from PFA preservation, safeguarding specimens for various rounds of processing.
Leverage the Very clear+ tissue clearing strategy, in conjunction with eFLASH and patented stochastic electrotransport systems, to fast clear and label whole organs. Crucial highlights and functions incorporate:
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